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Antibody-Oligonucleotide All-in-One Conjugation Kit

Cat.No.: A-9202-001
Size: Kit- conjugates 100ug of antibody
Price: $445.00
No chromatography required!  

The Application-Proven kit to conjugate antibodies with oligos just got even better.

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The Antibody-Oligonucleotide All-in-One Conjugation Kit offers an innovative TurboLink™ catalyzed conjugation based on Solulink's linking technology to prepare antibody-oligonucleotide conjugates without chromatography. This novel kit includes everything you needbuffers, spin columns, and a calculator to determine MSR. No column chromatography is required. With the Antibody-Oligonucleotide All-in-One Conjugation Kit, you can generate high-purity conjugates virtually free of residual antibody or oligonucleotide (>95% conjugate).

Download White Paper: Antibody-Oligonucleotide Conjugate Preparation and Applications

Workflow Diagram
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How it works
Solulink's linking technology is based on the use of two complementary heterobifunctional linkers:  

  1. Amine-modified, 20 to 60-mer oligonucleotide is modified using an excess of the Sulfo-S-4FB linker. This reactive NHS-ester incorporates a 4FB (aromatic aldehyde functional group, formylbenzamide) at the desired terminus of the oligonucleotide.
  2. Polyclonal or monoclonal antibody (100 μg) is modified using the S-HyNic linker. This NHS-ester reacts with lysine residues, incorporating HyNic functional groups (hydrazino-nicotinamide) onto the antibody.
  3. The two modified biomolecules are mixed together in the presence of the TurboLink™ catalyst, aniline, leading to rapid and efficient conversion of the antibody to conjugate through formation of stable bis-arylhydrazone bonds, followed by magnetic-affinity, solid phase purification.
  4. The antibody-oligonucleotide conjugate is ready for use in the most demanding and sensitive applications.

 

Advantages

  • Simple and Easy to Use – Requires only micropipette, microcentrifuge, and UV spectrophotometer
  • Automated Calculations – Calculator with fully integrated input/output provided
  • High Yield – 30–50% yield based on starting antibody
  • High Purity  >95% purity without chromatographic purification
  • High Stability – Conjugates are stable for >1 year

 Additional Information

Citations

  1. Noriyuki K.Kazuhiro K., Shinobu S., Yukitaka Y., Fusanori Y., Shingo M., Eisuke M. and Junichi E. Soluble heparin-binding EGF-like growth factor (HB-EGF) detected by a newly developed immuno-PCR method is a clear-cut serological biomarker for ovarian cancer. American Journal of Transitional Research, 2012, 4, 415-421.
  2. S. Fredriksson, M. Gullberg, J. Jarvius1, C. Olsson, K.Pietras, S.M Gustafsdottir, A. Ostman and U. Landegren, Protein detection using proximity-dependent DNA ligation assays, Nature Biotechnology 2002, 20, 473-477.
  3. Jiaming H., Tanyu W., Joonyul K., Curtis S., and Christopher E., Journal of the American Chemical Society, 2012, 134, 7066-7072.
  4. Schlingemann J, Leijon M, Yacoub A, Schlingemann H, Zohari S, Matyi-Tóth A, Kiss I, Holmquist G, Nordengrahn A, Landegren U. Novel means of viral antigen identification: Improved detection of avian influenza viruses by proximity ligation. Journal of virological methods 2010;163(1):116-122. Link
  5. Kamali-Moghaddam M, Pettersson E, Wu D, Englund H, Darmanis S, Lord A, Tavoosidana G, Sehlin D, Gustafsdottir S, Nilsson LNG. Sensitive detection of A-beta protofibrils by proximity ligation- relevance for Alzheimer's disease. BMC Neuroscience 2010;11(1):124. Link 
  6. Farrar D, Rai S, Chernukhin I, Jagodic M, Ito Y, Yammine S, Ohlsson R, Murrell A, Klenova E. Mutational analysis of the poly (ADP-ribosyl) ation sites of the transcription factor CTCF provides an insight into the mechanism of its regulation by poly (ADP-ribosyl) ation. Molecular and cellular biology 2010;30(5):1199. Link
  7. Darmanis S, Nong RY, Hammond M, Gu J, Alderborn A, Vänelid J, Siegbahn A, Gustafsdottir S, Ericsson O, Landegren U. Sensitive plasma protein analysis by microparticle-based proximity ligation assays. Molecular & Cellular Proteomics 2010;9(2):327. Link
  8. Liu G, Dou S, Chen X, Chen L, Liu X, Rusckowski M, Hnatowich DJ. Adding a clearing agent to pretargeting does not lower the tumor accumulation of the effector as predicted. Cancer Biother Radiopharm. 2010;25(6):757-62. Link  
  9. Kristjansdottir G, Sandling JK, Bonetti A, Roos IM, Milani L, Wang C, Gustafsdottir SM, Sigurdsson S, Lundmark A, Tienari PJ. Interferon regulatory factor 5 (IRF5) gene variants are associated with multiple sclerosis in three distinct populations. Journal of medical genetics 2008;45(6):362. Link
  10. Fredriksson a, Dixon W, Ji H, Koong AC, Mindrinos M, Davis RW. Multiplexed protein detection by proximity ligation for cancer biomarker validation. Nature Methods 2007;4(4):327-329. Link  
  11. Schallmeiner E, Oksanen E, Ericsson O, Spangberg L, Eriksson S, Ulf-Hakan S, Pettersson K, Landergren U. Sensitive protein detection via triple-binder proximity ligation assays. Nature Methods;2007;4(2):135-137. Link 
  12. Jarvius M, Paulsson J, Weibrecht I, Leuchowius KJ, Andersson AC, Wählby C, Gullberg M, Botling J, Sjöblom T, Markova B. In situ detection of phosphorylated platelet-derived growth factor receptor using a generalized proximity ligation method. Molecular & Cellular Proteomics 2007;6(9):1500. Link  


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