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ChromaLink™ Biotin (DMF Soluble)

Cat.No.: B-1001-010
Size: 10 mg
Price: $205.00
Cat.No.: B-1001-105
Size: 5 x 1.0 mg
Price: $175.00

Measurable biotinylation boosts reproducibility
ChromaLink™ Biotin contains a UV-traceable chromophore, based on Solulink's proprietary linking technology, to enable reproducibility in your biotinylation process. Now you can measure the degree of biotinylation in minutes, not days, without the standard curves required for HABA/avidin and fluoro-reporter assays. With a simple and direct UV scan, you can quantify biotin incorporation and ensure reproducible production of consistent batches. Pair up with Solulink's NanoLink™ Streptavidin Magnetic Beads (Cat. No. M-1002-010) for high-sensitivity ELISA, western blot, IHC, or immunoassay development.

  Download White Paper: How to Biotinylate with Quantifiable Results

ChromaLink™ Biotin Advantages

  • Reproducible Production UV-traceable chromophore permits nondestructive, rapid A280/A354 quantification of incorporated biotin, enabling consistency and reproducibility
  • Amine-Reactive – Contains an aromatic N-hydroxysuccinimide (NHS) ester reactive group, to efficiently couple to amines, such as the ε-amino group of lysine (K) or the N-terminus of the protein in aqueous systems
  • Extended PEG3 Spacer – Helps reduce aggregation, minimizes steric hindrance, and enhances solubility

Product Formats

  • ChromaLink™ Biotin Reagent – available in 5 × 1 mg and 10 mg sizes (Cat. No. B-1001-105 or B-1001-010)

Additional Information

Citations

  1. Sweetwyne MT, Pallero MA, Lu A, Van Duyn Graham L, Murphy-Ullrich JE. The Calreticulin-Binding Sequence of Thrombospondin 1 Regulates Collagen Expression and Organization During Tissue Remodeling. American Journal of Pathology 2010;177(4):1710-24. Link
  2. Sharma RK, Schabowsky RH, Srivastava AK, Elpek KG, Madireddi S, Zhao H, Zhong Z, Miller RW, MacLeod KJ, Yolcu ES, Shirwan H. 4-1BB Ligand as an Effective Multifunctional Immunomodulator and Antigen Delivery Vehicle for the Development of Therapeutic Cancer Vaccines. Cancer research 2010;70(10):3945. Link
  3. Dirksen A, Yegneswaran S, and Dawson PE. Bisaryl Hydrazones as Exchangeable Biocompatible Linkers.Angew. Chem. Int. Ed. 2010,49,2023–2027. Link
  4. Srinivasan B, Li Y, Jing Y, et al. A detection system based on giant magnetoresistive sensors and high-moment magnetic nanoparticles demonstrates zeptomole sensitivity: potential for personalized medicine.Angew. Chem. Int. Ed. Engl. 2009;48(15):2764-2767. Link
  5. Ma Z, Li Q, An H, Pankonin MS, Wang J, Loeb JA. Targeting Human Epidermal Growth Factor Receptor Signaling with the Neuregulin's Heparin-binding Domain. Journal of Biological Chemistry 2009;284(46):32108. Link

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