MHPH (Maleimide HyNic) Crosslinker
MHPH (Maleimide HyNic) is a maleimide that converts thiols on biomolecules and surfaces to HyNic linker molecules. The S-HyNic (succinimidyl-6-hydrazino-nicotinamide) heterobifunctional crosslinker is fundamental to Solulink’s proprietary linker technology. MHPH converts cysteine or thiol groups to HyNic linkers in a single step. The S-HyNic analog reacts with primary thiols on proteins (cysteine residues) introducing a HyNic (6-hydrazinonicotinamide) linker that forms stable covalent conjugates with biomolecules possessing 4FB (4-formylbenzamide) incorporated linkers. The advantages of the MHPH-4FB linker system include reaction specificity, UV-traceability, and the unique control it brings to the entire conjugation process.
Solulink’s proprietary linker technology is based on the use of two complementary heterobifunctional linkers:
- S-HyNic crosslinker or its analog is conjugated to biomolecule 1 through primary amines on proteins, oligos, peptides, or surfaces.
- S-4FB (succinimidyl-4-formylbenzamide) linker or its analog is conjugated to biomolecule 2 through primary amines on proteins, oligos, peptides, carbohydrates, or surfaces.
- HyNic-modified biomolecule 1 is incubated with 4FB-modified biomolecule 2 to form a conjugate.
The result is two biomolecules conjugated through a UV-traceable and stable bond (bis-arylhydrazone) with measurable absorbance at 354 nm. Thus any two proteins, regardless of molecular weight, can be efficiently conjugated.
- Catalyzed Conjugation – Faster kinetics for greater efficiency and yields
- Quantifiable – Conjugate bond is UV-traceable for simple and direct quantification
- Stability – 10 times more stable than any other conjugation linker
- Specificity – Two-linker method avoids homoconjugate formation
- Efficient – >80% efficient linker-biomolecule conjugations
- Robust - The conjugate bond is stable to 92˚C and to pH values ranging from pH 2.0–10.0
- Shen ZT, Brehm MA, Daniels KA, Sigalov AB, Selin LK, Welsh RM, Stern LJ. BI-specific MHC heterodimers for characterization of cross-reactive T cells. Journal of Biological Chemistry 2010;285(43):33144-53. Link
- Kwong GA, Radu CG, Hwang K, Shu CJ, Ma C, Koya RC, Comin-Anduix B, Hadrup SR, Bailey RC, Witte ON. Modular nucleic acid assembled p/MHC microarrays for multiplexed sorting of antigen-specific T cells. Journal of the American Chemical Society 2009;131(28):9695-9703. Link
- DeRouchey J, Schmidt C, Walker GF, Koch C, Plank C, Wagner E, Rädler JO. Monomolecular Assembly of siRNA and Poly (ethylene glycol)- Peptide Copolymers. Biomacromolecules 2008;9(2):724-732. <NO LINK>
- Levashova Z, Backer J, Backer M, Blankenberg F. Direct labeling of single-chain VEGF (sc-VEGF) with Tc99m. In: Society of Nuclear Medicine Annual Meeting Abstracts. Soc Nuclear Med; 2007 p. 181P. Link
Products and materials supplied by Solulink are for the purchaser’s internal research use only. Internal research use only for scientific investigation performed at the purchaser’s organization. Solulink material may not be used for any diagnostic, therapeutic or commercial use without a license from Solulink. Commercial purpose means any use for which you are compensated including any use in the manufacture of a product, information or data sale or any resale, repackaging or other transfer for consideration. Commercial licenses are available, please inquire.