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MTFB (Maleimide 4FB) Crosslinker

Cat.No.: S-1035-105
Size: 5 x 1.0 mg
Price: $210.00
Cat.No.: S-1035-010
Size: 10 mg
Price: $315.00

MTFB (Maleimide 4FB) is a maleimide that converts thiols on biomolecules and surfaces to 4FB linker molecules. The S-4FB (succinimidyl-4-formylbenzamide) heterobifunctional crosslinker is fundamental to Solulink's proprietary linker technology. MTFB converts cysteine or thiol groups to 4FB linkers in a single step. The S-4FB analog reacts with thiols on proteins (cysteine residues) introducing a 4FB (4-formylbenzamide) linker that forms stable covalent conjugates with biomolecules possessing HyNic (6-hydrazinonicotinamide) incorporated linkers. 4FB groups incorporated on biomolecules and surfaces are extremely stable and have been known to maintain their reactivity for >18 months. The advantages of the MTFB-4FB linker system include reaction specificity, UV-traceability, and the unique control it brings to the entire conjugation process.

Solulink's proprietary linker technology is based on the use of two complementary heterobifunctional linkers:  

  • S-HyNic crosslinker or its analog is conjugated to biomolecule 1 through primary amines on proteins, oligos, peptides, or surfaces.
  • S-4FB (succinimidyl-4-formylbenzamide) linker or its analog is conjugated to biomolecule 2 through primary amines on proteins, oligos, peptides, carbohydrates, or surfaces.
  • HyNic-modified biomolecule 1 is incubated with 4FB-modified biomolecule 2 to form a conjugate.

The result is two biomolecules conjugated through a UV-traceable and stable bond (bis-arylhydrazone) with measurable absorbance at 354 nm. Thus any two proteins, regardless of molecular weight, can be efficiently conjugated.

Solulink Advantages

  • Catalyzed Conjugation – Faster kinetics for greater efficiency and yields
  • Quantifiable – Conjugate bond is UV-traceable for simple and direct quantification
  • Stability – 10 times more stable than any other conjugation linker
  • Specificity – Two-linker method avoids homoconjugate formation
  • Efficient – >80% efficient linker-biomolecule conjugations
  • Robust - The conjugate bond is stable to 92˚C and to pH values ranging from pH 2.0–10.0

Additional Information

Products and materials supplied by Solulink are for the purchaser's internal research use only. Internal research use only for scientific investigation performed at the purchaser's organization. Solulink material may not be used for any diagnostic, therapeutic or commercial use without a license from Solulink. Commercial purpose means any use for which you are compensated including any use in the manufacture of a product, information or data sale or any resale, repackaging or other transfer for consideration. Commercial licenses are available, please inquire.