4FB/PEG3/Fluorescein

Fluorescently labeled antibodies, proteins, oligos or peptides can be used for flow cytometry, FISH, to monitor in vivo internalization, spatial distributions and/or localization. Solulink offers fluorescein dye pre-activated with aromatic aldehyde (4FB) group. 4FB/PEG3/Fluorescein is a 4FB-modified fluorescein fluorophore that is readily conjugated to any S-HyNic-modified antibody, protein, or peptide using Solulink’s linker technology.

Solulink’s proprietary linker technology is an innovative, catalyzed, UV-traceable, heterobifunctional linker technology that offers greater efficiency and yield in a considerably simpler method for linking any two biomolecules together e.g. proteins, oligos, or peptides regardless of molecular weight.

• S-HyNic (succinimidyl-6-hydrazino-nicotinamide) linker is conjugated to biomolecule 1 through primary amines (-NH₂) on the amino acid, lysine, or on the N-terminus.
• S-4FB (succinimidyl-4-formylbenzamide) linker is pre-conjugated to fluorescein fluorophore (Cat. No. S-4006)
• HyNic-modified biomolecule 1 is incubated with 4FB/PEG3/Fluorescein in a catalyzed conjugation reaction.

The result is two biomolecules conjugated through a UV-traceable, stable bond (bis-arylhydrazone) with measurable absorbance at 354 nm. Solulink’s efficient 4FB-HyNic conjugation chemistry permits labeling at low dye-to-protein reaction ratios. This labeling efficiency guarantees greater control over the labeling reaction and reduces non-specific adsorption of fluorescent conjugates often seen with less controlled (higher) dye to protein substitution ratios.

Advantages

• Not susceptible to hydrolysis unlike NHS-esters of fluorescein
• Desalting of HyNic-modified proteins not required prior to labeling with 4FB/PEG3/Fluorescein

Additional Information

• Product Data Sheet/Certificate of Analysis
• MSDS

Products and materials supplied by Solulink are for the purchaser’s internal research use only. Internal research use only for scientific investigation performed at the purchaser’s organization. Solulink material may not be used for any diagnostic, therapeutic or commercial use without a license from Solulink. Commercial purpose means any use for which you are compensated including any use in the manufacture of a product, information or data sale or any resale, repackaging or other transfer for consideration. Commercial licenses are available, please inquire.