MES Buffer (10X)
|Size:||5 x 1.5 mL|
MES is used as a buffering agent in biology and biochemistry, for applications such as protein electrophoresis. At Solulink, MES Buffer is used to prepare the 2-sulfobenzaldehyde (2-SBA) assay reagent for HyNic MSR quantification and in conjugating HyNic-modified peptides to NanoLink 4FB Magnetic Beads.
Solulink's proprietary linker technology is an innovative, catalyzed, UV-traceable, heterobifunctional linker technology that offers greater efficiency and yield in a considerably simpler method for linking any two biomolecules together e.g. proteins, oligos, peptides or surfaces.
- S-HyNic (succinimidyl-6-hydrazino-nicotinamide) linker is conjugated to biomolecule 1 through primary amines (-NH2) on the amino acid, lysine, or on the N-terminus.
- S-4FB (succinimidyl-4-formylbenzamide) linker is conjugated to biomolecule 2 through primary amines (-NH2) on the amino acid, lysine, or on the N-terminus.
- HyNic-modified biomolecule 1 is incubated with 4FB-modified biomolecule 2 in a catalyzed conjugation reaction.
The result is two biomolecules conjugated through a UV-traceable, stable bond (bis-arylhydrazone) with measurable absorbance at 354 nm.
- Allophycocyanin (APC)-Antibody Conjugation Kit (Cat. No. P-9903-001)
- R-PE-Antibody Conjugation Kit (Cat. No. P-9002-002)
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