All Purpose Crosslinking Kit

The All Purpose Crosslinking Kit provides the fundamentals - S-HyNic and S-4FB - of Solulink’s proprietary linker technology, offering greater efficiency and yield in a considerably simpler conjugation method. Use S-HyNic and S-4FB to easily link together any two proteins, peptides, oligos, beads, or surfaces.

Solulink’s proprietary linker technology is based on the use of two complementary heterobifunctional linkers:  

Workflow Diagram
click to expand

• S-HyNic (succinimidyl-6-hydrazino-nicotinamide) linker is conjugated to biomolecule 1 through primary amines (-NH₂) on proteins, oligos, peptides, carbohydrates, or surfaces.
• S-4FB (succinimidyl-4-formylbenzamide) linker is conjugated to biomolecule 2 through primary amines (-NH₂) on proteins, oligos, peptides, carbohydrates, or surfaces.
• HyNic-modified biomolecule 1 is incubated with 4FB-modified biomolecule 2 to form conjugate.

The result is two biomolecules conjugated through a UV-traceable, stable bond (bis-arylhydrazone) with measurable absorbance at 354 nm. Any two proteins, regardless of molecular weight, can be efficiently conjugated.

All Purpose Crosslinking Kit Advantages

• Versatile – Linkers connect any two biomolecules together
• Quantifiable – Using a UV signature wavelength and simple UV scan
• Stable – 10 times more stable than any other conjugation linker
• Specificity – Two-linker method avoids homoconjugate formation

Additional Information

• Manuals and Protocol
• Certificate of Analysis
• MSDS

Citations

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