ChromaLink™ Biotin Protein Labeling Kit
| Cat #: | B-9007-105K |
| Size: | Kit- labels 25 µg to 1 mg |
| Price: | $395.00 |
Convenient kit boosts biotinylation reproducibility
The ChromaLink™ Biotin Labeling Kit provides everything you need for convenient, measurable, antibody or protein biotinylation. The kit contains ChromaLink™ Biotin, which incorporates a UV-traceable chromophore, based on Solulink's proprietary linking technology, to enable reproducibility in your biotinylation process. Now you can measure the degree of biotinylation in minutes, not hours (HABA), without the standard curves required for HABA/avidin and fluoro-reporter assays. With a simple and direct UV scan (A280 and A354), you can quantify biotin incorporation and ensure reproducible production of consistent batches. A large scale kit is also available (labels 2 mg of antibody).
Workflow Diagram
click to expand
Download White Paper: How to Biotinylate with Quantifiable Results
The ChromaLink™ Biotin Labeling Kit includes everything you need to biotinylate your antibody: buffers, reagents, spin columns, and an easy-to-follow protocol. With the ChromaLink™ Biotin Labeling Kit, you can biotinylate and purify up to 5 antibodies or proteins ranging in molecular weight from 20–200 kDa from as little as 25 µg to as much as 1 mg in about 2.5 hours. The resulting biotinylated antibody is ready for binding to streptavidin. We recommend our NanoLink™ Streptavidin Magnetic Beads (Cat. No. M-1002-010) with the highest biotin-binding capacity available for assay development.
ChromaLink™ Biotin Labeling Kit Advantages
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Reproducible Production – UV-traceable chromophore permits nondestructive, rapid A280/A354 quantification of incorporated biotin, enabling consistency and reproducibility
Extended PEG3 Spacer – Helps reduce aggregation, minimizes steric hindrance, and enhances solubility
Scalable – Labels 25 µg to 1 mg of antibody
Convenient – Kit contains buffers, spin columns, and calculator to determine reaction quantities
Solulink ChromaLink™ Biotin Labeling Kit |
Pierce EZ-Link Sulfo-NHS-Biotinylation Kit |
|
Biotinylation Time |
2.5 hours |
2.5 hours |
Quantification of Biotin |
5 minute UV Scan = Free |
3 hour HABA Assay = $140 |
Solulink ChromaLink™ Biotin Labeling Kit |
Pierce EZ-Link Sulfo-NHS-Biotinylation Kit |
Life Technologies APEX™ Biotin-XX Antibody Labeling Kit |
|
Biotin Quantification |
Yes |
No |
No |
Complete Kit |
Yes |
No |
No |
Purification |
Yes |
No |
No |
Additional Information
- Protocol
- Calculators (Labeling and MSR)
- Certificate of Analysis
- Shipping and Storage Information
- MSDS
Citations
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Sweetwyne MT, Pallero MA, Lu A, Van Duyn Graham L, Murphy-Ullrich JE. The Calreticulin-Binding Sequence of Thrombospondin 1 Regulates Collagen Expression and Organization During Tissue Remodeling. American Journal of Pathology 2010;177(4):1710-24. Link
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Sharma RK, Schabowsky RH, Srivastava AK, Elpek KG, Madireddi S, Zhao H, Zhong Z, Miller RW, MacLeod KJ, Yolcu ES, Shirwan H. 4-1BB Ligand as an Effective Multifunctional Immunomodulator and Antigen Delivery Vehicle for the Development of Therapeutic Cancer Vaccines. Cancer research 2010;70(10):3945. Link
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Dirksen A, Yegneswaran S, and Dawson PE. Bisaryl Hydrazones as Exchangeable Biocompatible Linkers.Angew. Chem. Int. Ed. 2010,49,2023–2027. Link
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Srinivasan B, Li Y, Jing Y, et al. A detection system based on giant magnetoresistive sensors and high-moment magnetic nanoparticles demonstrates zeptomole sensitivity: potential for personalized medicine.Angew. Chem. Int. Ed. Engl. 2009;48(15):2764-2767. Link
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Ma Z, Li Q, An H, Pankonin MS, Wang J, Loeb JA. Targeting Human Epidermal Growth Factor Receptor Signaling with the Neuregulin's Heparin-binding Domain. Journal of Biological Chemistry 2009;284(46):32108. Link
Sweetwyne MT, Pallero MA, Lu A, Van Duyn Graham L, Murphy-Ullrich JE. The Calreticulin-Binding Sequence of Thrombospondin 1 Regulates Collagen Expression and Organization During Tissue Remodeling. American Journal of Pathology 2010;177(4):1710-24. Link
Sharma RK, Schabowsky RH, Srivastava AK, Elpek KG, Madireddi S, Zhao H, Zhong Z, Miller RW, MacLeod KJ, Yolcu ES, Shirwan H. 4-1BB Ligand as an Effective Multifunctional Immunomodulator and Antigen Delivery Vehicle for the Development of Therapeutic Cancer Vaccines. Cancer research 2010;70(10):3945. Link
Dirksen A, Yegneswaran S, and Dawson PE. Bisaryl Hydrazones as Exchangeable Biocompatible Linkers.Angew. Chem. Int. Ed. 2010,49,2023–2027. Link
Srinivasan B, Li Y, Jing Y, et al. A detection system based on giant magnetoresistive sensors and high-moment magnetic nanoparticles demonstrates zeptomole sensitivity: potential for personalized medicine.Angew. Chem. Int. Ed. Engl. 2009;48(15):2764-2767. Link
Ma Z, Li Q, An H, Pankonin MS, Wang J, Loeb JA. Targeting Human Epidermal Growth Factor Receptor Signaling with the Neuregulin's Heparin-binding Domain. Journal of Biological Chemistry 2009;284(46):32108. Link
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