RapidDirect™ Primary Antibody polyHRP IP/Western Blot Kits

For IP/western blots. Modify your primary antibody directly with HRP. No purification. No secondary antibodies needed. Ideal for those who use the same antibody for multiple western blots with the additional benefit of time savings. 

Download Application Note: How to Get Publication-Quality Western Blots

Solulink's RapidDirect Primary Antibody polyHRP IP/Western Blot Kit advantages:

Workflow Diagram
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Solulink's  RapidDirect Primary Antibody polyHRP IP/Western Blot Kit uses direct labeling of HRP to the anti-antigen antibody in near quantitative yield, producing a highly sensitive one-step detection reagent. This eliminates the root cause of the interfering bands resulting from binding of detecting reagents to heavy and light chains of antibodies and other proteins on the blot membrane.

This kit converts 75-100 µg of primary antibody to primary antibody polyHRP conjugate, requiring only pipettes and a microcentrifuge in <30 minutes hands-on-time. The final conjugate allows at minimum development of 10-40 western blots dependent on the inherent affinity of the primary antibody. Examples of western blots from both mouse LLC and cell lysates are presented in the manual.

• Protocol
  
• Product Data Sheet
  
• MSDS