Is there a limit to the size of my oligo?
Yes. The Antibody-Oligonucleotide Conjugation Kit is designed to conjugate oligos between 20 and 60 bases though some users have used oligos up to 120 bases. For oligos outside of this size range please contact our technical support team for advice.
Does my oligo have to be HPLC purified?
Yes. The oligo MUST be HPLC purified due to oligo synthesis reactants interfering with the conjugation chemistry.
Does Solulink offer a custom oligo conjugation service?
Yes. Solulink does offer a custom oligo conjugation service. Please contact our technical support team or phone +1.858.625.0670.
Do I need specific functional groups on my Oligo?
Yes. The oligo must contain a terminal amine or 4FB group. This amine group is added during oligo synthesis and may be either 5’ or 3’. The 4FB can be added during oligo synthesis by our partner TriLink Biotechnologies at this link: http://www.trilinkbiotech.com/cart/scripts/prodView.asp?idproduct=6010
Can I use the kit to conjugate oligos to proteins other than antibodies?
No, Solulink’s Antibody-Oligo Conjugation Kit is primarily designed to conjugate oligos to purified antibodies. However, Solulink does have a Protein-Oligo Kit that does two conjugations S-9011-1 which can be found at this link: http://store.solulink.com/products/s-9011-1-protein-oligonucleotide-conjugation-kit or please contact our technical support team for specific advice.
Does antibody species or isotype make a difference to the conjugation efficiency?
No. The conjugation system is primarily designed to conjugate antibodies to purified IgG. The kit will conjugate oligos to IgG irrespective of species. The kit will also conjugate all other antibody sub types. Please contact our technical support team for specific advice.
Can I use the Solulink Antibody-Oligo Kit to conjugate double stranded DNA or RNA to antibodies?
Yes. It works best with DNA or RNA of <60bp. For advice and support on conjugating other forms of nucleic acids please contact our technical support team.
Are larger pack sizes available?
Yes. The standard kit is designed to conjugate 100μg of antibody. Solulink can provide larger kits on request. Please contact our sales team.