2- Sulfobenzaldehyde

Cat.No.: S-2005-100
Size: 100 mg
Price: $183.00

2-Sulfobenzaldehyde is a water-soluble aromatic aldehyde reagent used to quantify the molar substitution ratio (MSR) of HyNic-modified proteins or other biomolecules. This aromatic aldehyde reacts with HyNic-modified biomolecules and forms a quantifiable hydrazone absorbance signature at 345 nm (molar extinction coefficient @ 345 nm = 28,500). This signature is used to determine the number of HyNic groups incorporated in a protein or other biomolecule after modification with S-HyNic. S-HyNic-modified proteins or biomolecules can be conjugated to S-4FB-modified biomolecules using Solulink's proprietary linker technology.

Solulink's proprietary linker technology is an innovative, catalyzed, UV-traceable, heterobifunctional linker technology that offers greater efficiency and yield in a considerably simpler method for linking any two biomolecules together e.g. proteins, oligos, or peptides regardless of molecular weight.

  • S-HyNic (succinimidyl-6-hydrazino-nicotinamide) linker is conjugated to biomolecule 1 through primary amines (-NH2) on the amino acid, lysine, or on the N-terminus.
  • S-4FB (succinimidyl-4-formylbenzamide) linker is conjugated to biomolecule 2 through primary amines (-NH2) on the amino acid, lysine, or on the N-terminus.
  • HyNic-modified biomolecule 1 is incubated with 4FB-modified biomolecule 2 in a catalyzed conjugation reaction.

The result is two biomolecules conjugated through a UV-traceable, stable bond (bis-arylhydrazone) with measurable absorbance at 354 nm. 

Additional Information

Products and materials supplied by Solulink are for the purchaser's internal research use only. Internal research use only for scientific investigation performed at the purchaser's organization. Solulink material may not be used for any diagnostic, therapeutic or commercial use without a license from Solulink. Commercial purpose means any use for which you are compensated including any use in the manufacture of a product, information or data sale or any resale, repackaging or other transfer for consideration. Commercial licenses are available, please inquire.